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What exactly is the difference between agarose gel 4B, 6B, 4FF, etc.?

2025/12/30 Viewers:

Agarose gels are linear polysaccharides formed by alternating β-D-galactose and 3,6-dehydrated-L-galactose. The purified agarose thermal solution can be gelled when cooled, and beads can be easily made. In the gelling process, a double helix is formed first by individual polysaccharide chains, and then aggregates into micelles, between which micropores are formed, the size of which depends on the number of micelles, i.e. the concentration of agarose. Using different concentrations of agarose solution, spherical gels with different pore sizes can be obtained after spheroidization. Final gel structure = agarose gel structure.


Sepharose is the earliest product in the agarose system, which is a non-cross-linked structure, cannot be autoclaved, and is only used in the range of 2~40°C, and is divided into three types of 2B, 4B and 6B according to the agarose content of 2%, 4% and 6%, which can be used to separate nucleotide compounds.


When agarose gel is covalently cross-linked with 2,3-dibromopropanol, the thermal stability and physicochemical stability of sepharose CL can be greatly improved, and it can be sterilized at 120°C under neutral conditions and has small non-specific adsorption, and is divided into three types of CL-2B, CL-4B and CL-6B according to the agarose content of 2%, 4% and 6%, which are widely used as ion exchangers and affinity sorbents in addition to gel filtration.

Agarose gel 4FF and 6FF are highly coupled agarose gels, which have greatly enhanced mechanical properties and fast flow rates, making them suitable for industrial preparation and production. It has high chemical stability and can be operated with a variety of solvent-promoting and organic solvents and cleaned by 1~2M NaOH in situ.


Agarose gel medium: 4B (4% agarose)-CL-4B (low-strength cross-linking)-4FF (high-strength cross-linking), the rigidity is gradually enhanced, and the pressure resistance is improved, but in the process of cross-linking, some groups have been occupied, so the ligands that can be cross-linked gradually decrease, and the density of the ligands of the prepared medium gradually decreases.