Sagarose^® low electrical endoosmotic agarose

Agarose with low EEO

1. Product description

Sagarose^® agarose series products are equipped with innovative second-generation desulfurization technology, breaking through the shackles of natural seaweed raw materials and realizing the free regulation of core indicators such as gel strength and electroostosis through molecular-level fine control processes. Magarose^® Low Electroosmotonic Agarose is a standard gel temperature, high gel strength agarose suitable for separating DNA fragments from 100 bp to 15,000 bp. Designed for preparative DNA electrophoresis, this product is a genetically technological-grade agarose that has been thoroughly tested for performance to ensure compatibility with conventional molecular biology techniques.

2. Basic parameters

3. Product specifications

4. How to use

(1) Optimal separation range of agarose gel preparation concentration from DNA

In the process of agarose gel electrophoresis, the appropriate agarose concentration and electrophoresis buffer should be selected according to the molecular weight of the nucleic acids to be separated by electrophoresis, so as to improve the resolution of nucleic acid molecules. Agarose gel concentration and linear DNA isolation range and electrophoresis solution refer to the following table:

(2) Experimental steps

1. Prepare the corresponding concentration of agarose solution with TAE/TBE, microwave until boiling, put on heat-resistant gloves, and carefully shake the conical flask to dissolve the agarose gum until it is clear and transparent.

2. Add the dye to the hot agarose solution according to the recommended concentration of nucleic acid dye. Mix well and pour into the pre-prepared glue mold. Insert a comb in place and wait for the glue to fully solidify at room temperature or refrigerated conditions. Gel thickness is generally between 3-5 mm.

3. Load the gel in an electrophoresis tank (reference condition: constant pressure 125 V, 35-40 min).

5. Precautions

1. This product is only for scientific research use and should not be used in clinical diagnosis and treatment.

2. When this product is melted by microwave heating, please wear goggles and insulated gloves to protect yourself and others from burns.

3. The dissolution time of this product can be referred to: 100 mL, 1% concentration agarose recommended microwave oven high heat heating time: AG1400LE-50 s。

5. Precautions

1. This product is only for scientific research use and should not be used in clinical diagnosis and treatment.

2. When dissolving in the microwave, try to minimize the heating time, and always wear goggles to avoid burns.

6. Transportation and preservation methods

7. Related product recommendations

8. Application cases

(1) Nucleic acid electrophoresis examples at different gel concentrations