Sagarose^® High Resolution Agarose

Agarose with high resolution

1. Product description

Sagarose^® High Resolution Agarose has a medium melting temperature that is twice the resolution of traditional sieved agarose products. Horizontal gel electrophoresis resolves PCR products and small DNA fragments with only 2% difference in size. It can be applied to the high-resolution separation of DNA fragments from 20 bp to 700 bp; Recovery of fragments below 700 bp; Fine analysis of PCR products; AMPFLP, STR and tertiary and tetranucleotide repeat analysis.

2. Basic parameters

3. Product information

4. How to use

(1) Optimal separation range of agarose gel preparation concentration from DNA

In the process of agarose gel electrophoresis, the appropriate agarose concentration and electrophoresis buffer should be selected according to the molecular weight of the nucleic acids to be separated by electrophoresis, so as to improve the resolution of nucleic acid molecules. Agarose gel concentration and linear DNA isolation range and electrophoresis solution are referred to the following table:

Procedure:

• Choose a beaker with a capacity of 2-4 times the volume of the solution and add pre-cooled 1× or 0.5 × of electrophoresis buffer and stirrer.

• Sprinkle in the agarose powder slowly while stirring quickly, and soak the agarose in the buffer for 15 minutes before heating to reduce foam formation when heating.

• Weigh the beaker and the total weight of the solution before heating, cover the beaker with plastic wrap, and make small holes in the plastic wrap for venting.

Note: If the agarose concentration >4%, the following steps should be performed to prevent foaming
A. Microwave over medium heat for 1 minute. B. Remove the solution and let stand for 5 minutes.

• Microwave over medium heat for 2 minutes and remove the beaker.

Note: Microwaved solutions may overheat and splash when shaken.

• Gently rotate the beaker to resuspend the settled powder or gel block.

• Heat over high heat until the solution boils, keeping boiling for 1 minute or until all particles are completely dissolved.

• Remove the beaker and gently rotate the beaker to mix the solution well.

• After dissolution, add an appropriate amount of hot water to the initial weight and mix well.

• After cooling to 50 °C-60 °C, the glue is poured, the gel is solidified at room temperature, and then placed in an electrophoresis tank for electrophoresis (recommended parameters: 4% AG0070HR, constant pressure 90 V, 70-80 min).

5. Precautions

1. This product is only for scientific research use and should not be used in clinical diagnosis and treatment.

2. Always wear goggles to protect yourself and others from burns when microwaving to dissolve agarose.

3. The gel strength of this product is low, please place it on a hard plate and transfer.

6. Transportation and preservation methods

7. Related product recommendations

 

 

8. Application cases