Magarose^®3:1 agarose

3:1 Agarose with high resolution

1. Product description

Magarose^® 3:1 agarose is a low-melting temperature agarose suitable for isolating DNA fragments ≤ 5,000 bp. Its high gel transparency and gel strength make it easy to handle the gel, enhancing the convenience of gel handling and blotting. Performance tested, Magarose^® 3:1 agarose ensures fine resolution of DNA and RNA fragments ≤ 5,000 bp for PCR product separation and blotting experiments.

2. Basic parameters

3. Product information

4. How to use

(1) Optimal separation range of agarose gel preparation concentration from DNA

In the process of agarose gel electrophoresis, the appropriate agarose concentration and electrophoresis buffer should be selected according to the molecular weight of the nucleic acids to be separated by electrophoresis, so as to improve the resolution of nucleic acid molecules. Agarose gel concentration and linear DNA isolation range and electrophoresis solution refer to the following table:

(2) Experimental steps

• Choose a beaker with a capacity of 2-4 times the volume of the solution and add pre-cooled 1× or 0.5 × of electrophoresis buffer and stirrer.

• Sprinkle in the agarose powder slowly while stirring quickly, and soak the agarose in the buffer for 15 minutes before heating to reduce foam formation when heating.

• Weigh the beaker and the total weight of the solution before heating, cover the beaker with plastic wrap, and make small holes in the plastic wrap for venting.

Note: If the agarose concentration >4%, the following steps should be performed to prevent foaming
A. Microwave over medium heat for 1 minute. B. Remove the solution and let stand for 5 minutes.

• Microwave over medium heat for 2 minutes and remove the beaker.

Note: Microwaved solutions may overheat and splash when shaken.

• Gently rotate the beaker to resuspend the settled powder or gel block.

• Heat over high heat until the solution boils, keeping boiling for 1 minute or until all particles are completely dissolved.

• Remove the beaker and gently rotate the beaker to mix the solution well.

• After dissolution, add an appropriate amount of hot water to the initial weight and mix well.

• After cooling to 50 °C-60 °C, the glue is poured to solidify at room temperature, and then placed in an electrophoresis tank for electrophoresis (recommended parameters: constant pressure 70-90 V, 40-60 min).

5. Precautions

1. This product is only for scientific research use and should not be used in clinical diagnosis and treatment.

2. When dissolving in the microwave, try to minimize the heating time, and always wear goggles to avoid burns.

6. Transportation and preservation methods

7. Related product recommendations

 

8. Application cases

(1) Nucleic acid electrophoresis examples at different gel concentrations